Quantification of PERF 15 mRNA in Tissue Sections from Rat Testes
نویسندگان
چکیده
منابع مشابه
Quantification of PERF 15 mRNA in Tissue Sections from Rat Testes
We previously conducted basic research to quantify in situ hybridization (ISH) signals in rat testes. In this experimental model, we selected ribosomal RNA (rRNA) as the hybridizable RNA in paraffin sections, since it allowed us to easily analyze ISH signals expressed with digoxygenin (DIG)-labeled probes quantitatively through "posterization" of the images. We applied this method to analyze th...
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Thirty years of literature have shown that changes in mRNA abundance provide insight into cellular functioning. Indeed, mRNA abundance measurements have been used to determine how effective particular drugs are in eliciting cellular responses, in monitoring behavioral responses, and in several other areas of neurobiology. Although investigation of individual mRNAs was informative, there are man...
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Background: Chronic diabetes mellitus is accompanied with enhanced oxidative stress and reduce the activity of antioxidant defense system. Due to significant role of enhanced oxidative stress in development of testes damage in diabetices, this study was conducted to evaluate the effect of royal jelly on oxidative stress marker in testis tissue of diabetic rats. Materials and Methods: In this ex...
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Measurement of mRNA levels across tissue samples facilitates an understanding of how genes function and what their roles are in disease. Quantifying low-abundance mRNA requires a workflow that preserves spatial information, isolates RNA, and performs reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR). This is complex because these steps are typically performed in three separ...
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The aim of the present study was stereological evaluation of testes of azoospermic animal model using busulfan in rat. Three groups of male adult rats were used in this study. The first group was injected by single dose of busulfan (10 mg kg-1) and their testes were removed on day 35 post injection. The second group received double doses of busulfan with 21 days interval and their te...
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 2006
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.06016